陈剑平科研成就科研综述陈剑平首次在禾谷多黏菌体内发现大麦和性花叶病毒和大麦黄花叶病毒,为真菌传播植物病毒提供了直接证据,揭示了真菌与其传播病毒的内在关系
针对中国三农问题综合解决方案,创新提出了现代农业综合体作为区域现代农业发展创新载体的建设背景、科学内涵、理论基础、典型案例和建设机制
截至2016年6月,陈剑平揭示了5种重要稻麦病毒发生规律和发病机理,建立的病害绿色防控技术在中国病区大面积推广应用;建立了一大批先进的检测技术,鉴定了数十种植物病害新病原,截获了上千批次外来入侵植物病原
建立了500余种花卉、中药材无病毒组培快繁产业化技术,开发了25种花卉组培苗新产
承担项目截至2018年11月,陈剑平先后承担欧盟、国家高新技术研究和发展计划(863)、国家重点基础研究发展计划(973)和国家转基因生物新品种培育科技重大专项等40多个研究项目
学术论著截至2018年11月 ,陈剑平发表论文360多篇(其中SCI收录论文180余篇),主编专著10部、参编4部 ,授权发明专利52件,软著4件
主要论文:1.Yang J, Zhang HM*, Ying L, Li J, Lv MF, Xie L, Li PP, Liu XY, DL, Chen JP*.Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants.Archive of Virology,2014,159:307-3142.Xue J, Li J, Zhan HM*, Yang J, Lv MF, Gao BD, Chen JP*.Molecular characterization of Southern rice blacked-streaked dwarf virus (SRBSDV) from Vietnam.Journal of Phytopathology,2014,162(6):349-3583.Chen M, Sun LY, Wu HY, Chen J, Ma YZ, Zhang XX, Du LP, Cheng SH, Zhang BQ, Ye XG, Pang JL, Zhang XM, Li LC, Andika IB, Chen JP*, Xu HJ*.Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the anti-sense virus polymerase gene.Plant Biotechnology Journal,2014,12(4):447–4564.Li JM, Andika IB, Zhou YR, Shen JF, Sun ZT, Wang X, Sun LY*, Chen JP*.Unusual characteristics of dicistrovirus-derived small RNAs in the small brown planthopper, Laodelphax striatellus.Journal of General Virology,2014,95:712-7185.Zhou J, Yu FB, Wang XM, Yang Y, Yu CL, Liu HJ, Cheng Y, Yan CQ, Chen JP*.Specific expression of DR5 promoter in rice roots using a tCUP derived promoter-reporter system.PloS One,2014,9(1):e870086.Sun LY, Andika IB, Shen JF, Yang D, Chen JP*.The P2 of Wheat yellow mosaic virus rearranges the endoplasmic reticulum and recruits other viral proteins into replication-associated inclusion bodies.Molecular Plant Pathology,2014,15(5):466-4787.Rong LL, Lu YW, Lin L, Zheng HY, Yan F, Chen JP*.A transmembrane domain determines the localization of rice stripe virus pc4 to plasmodesmata and is essential for its function as a movement protein.Virus research,2014,183:112-1168.Yan F*, Guo WX, Wu GT, Lu YW, Peng JJ, Zheng HY, Lin L, Chen JP*.A virus-based miRNA Suppression (VbMS) system for miRNA loss-of-function analysis in plants.Biotechnology Journal,2014,9(5):702-89.Wu GT, Wang JY, Yang Y, Dong B, Wang YL, Sun GC, Yan CQ, Yan F*, Chen JP*.Transgenic rice expressing rice stripe virus NS3 protein, a suppressor of RNA silencing, shows resistance to rice blast disease.Virus Genes,2014,48(3):566-910.Zhang WL, Dong Y, Yang L, Ma BJ, Ma RR, Huang FD, Wang CC, Hu HT, Li CS, Yan CQ*, Chen JP*.Small brown planthopper resistance loci in wild rice (Oryza officinalis).Molecular Genetics and Genomics,2014,289(3):373-8211.Han XY, Yang Y, Wang XM, Zhou J, Zhang WH, Yu CL, Cheng C, Cheng Y, Yan CQ*, Chen JP*.Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis.International Journal of Molecular Sciences,2014,15:11847-1186112.Yang J, Zhang HM*, Liu XY, Li J, Lv MF, Li PP, Dai LY, Chen JP*.Identification of 23 novel conserved microRNAs in three rice cultivars.Gene,2014,548(2):285-29313.Andika IB, Zheng SL, Tan ZL, Sun LY*, Kondo H, Zhou XP, Chen JP*.Endoplasmic reticulum export and vesicle formation of the movement protein of Chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway.Virology,2013,435(2):493-50314.Sun LY, Andika IB*, Kondo H, Chen JP*.Identification of the amino acid residues and domains in the cysteine-rich protein of Chinese wheat mosaic virus that are important for RNA silencing suppression and subcellular localization.Molecular Plant Pathology,2013,14(3):265-27815.Sun ZT, Zhang SL, Xie L, Zhu QS, Tan ZL, Bian J, Sun LY, Chen JP*.The secretory pathway and the actomyosin motility system are required for plasmodesmatal localization of the P7-1 of Rice black-streaked dwarf virus.Archive of Virology,2013,(158):1055-106416.Wang XM, Zhou J, Yang Y, Yu FB, Chen J, Yu CL, Wang F, Cheng Y, Yan CQ, Chen JP*.Transcriptome Analysis of a Progeny of Somatic Hybrids of Cultivated Rice (Oryza sativa L.) and Wild Rice (Oryza meyeriana L.) With High Resistance to Bacterial Blight.Journal of Phytopathology,2013,161(5):324-33417.Wang XM, Chen J, Yang Y, Zhou J, Qiu Y, Yu CL, Cheng Y, Yan CQ, Chen JP*.Characterization of a novel NBS-LRR gene involved in bacterial Blight resistance in Rice.Plant Molecular Biology Reporter,2013,31(3):649-65618.Li J, Xue J, Zhang HM*, Yang J, Lv MF, Xie L, Meng Y, Li PP, Chen JP*.Interactions between the P6 and P5-1 proteins of southern rice black-streaked dwarf fijivirus in yeast and plant cells.Archive of Virology,2013,158(8):1649-165919.Wu WQ, Guo XG, Zhang HM*, Yang J, Lv MF, Chen JP*.Simultaneous detection and survey of three rice viruses in China.Plant disease,2013,97(9):1181-118620.Zhou J, Yu FB, Chen B, Wang XM, Yang Y, Cheng Y, Yan CQ, Chen JP*.Universal vectors for constructing artificial microRNAs in plants.Biotechnol Letter,2013,35(7):1127-3321.Zhou J, Yang Y, Wang XM, Yu FB, Yu CL, Chen J, Cheng Y, Yan CQ*, Chen JP*.Enhanced transgene expression in rice following selection controlled by weak promoters.BMC Biotechnology,2013,13:2922.Sun BJ, Sun LY, Tugumed AK, Adamse MJ, Yang J, Xie LH, Chen JP*.Selection pressure and founder effects constrain genetic variation in differentiated populations of a soil-borne bymovirus Wheat yellow mosaic virus (Potyviridae) in China.Phytopathology,2013,103:949-95923.Xue J, Li J, Ta HA, Zhang HM*, Yang J, Lv MF, Meng Y, Li PP, Chen JP*.The complete genomic sequence of Southern rice blacked-draw virus, a novel fijivirus, from Vietnam.Genome Announcements,2013,1(3):e00212-1324.Wu GT, Lu YW, Zheng HY, Lin L, Yan F*, Chen JP*.Transcription of ORFs on RNA2 and R NA4 of Rice stripe virus terminate at an AUCCGGAU sequence that is conserved in the genus Tenuivirus .Virus Research,2013,175(1):71-725.Sun LY*, Xie L, Andika IB, Tan ZL, Chen JP*.Non-structural protein P6 encoded by rice black-streaked dwarf virus is recruited to viral inclusion bodies by binding to the viroplasm matrix protein P9-1.Journal of General Virology,2013,94(8):1908-1626.Li JM, Andika IB, Shen JF, Lv YD, Ji YQ, Sun LY*, Chen JP*.Characterization of Rice Black-Streaked Dwarf Virus- and Rice Stripe Virus-Derived siRNAs in Singly and Doubly Infected Insect Vector Laodelphax striatellus.PLoS ONE,2013,8(6):e6600727.Sun LY, Andika IB*, Shen JF, Yang D, Ratti C, Chen JP*.The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.Virus Research,2013,177:66-7428.Yu CL, Karlin DG, Lu YW, Wright K, Chen JP, MacFarlane S*.Experimental and bioinformatic evidence that raspberry leaf blotch emaravirus P4 is a movement protein of the 30K superfamily.Journal of General Virology,2013,94:2117-212829.Sun ZT, Yang D, Xie L, Sun LY, Zhang SL, Zhu QS, Li JM, Wang X, Chen JP* .Rice black-streaked dwarf virus P10 induces membranous structures at the ER and elicits the unfolded protein response in Nicotiana benthamiana.Virology,2013,447:131-13930.Sun LY*, Bian J, Andika IB, Hu YC, Sun BJ, Xiang R, Hideki Kondo, Chen JP*.Nucleo-cytoplasmic shuttling of VPg encoded by wheat yellow mosaic virus requires association with the coat protein.Journal of General Virology,2013,94:2790-280231.Dong RX, Chen J, Wang XM, Li JS, Zhou J, Yang Y, Yu CL, Cheng Y, Yan CQ, Chen JP*.Agrobacterium-mediated transformation efficiency is altered in a novel rice bacterial blight resistance cultivar and is influenced by environmental temperature.Physiological and Molecular Plant Pathology,2012,77:33-4032.He QJ, Peng JJ, Yan F, Lin L, Lu YW, Zheng HY, Chen JP*.Intron retention and 3`-UTR analysis of Arabidopsis Dicer-like 2 transcripts.Molecular biology reports,2012,39:3271-328033.Yan F, Lu YW, Wu GT, Peng JJ, Zheng HY, Lin L, Chen JP*.A simplified method for constructing artificial microRNAs based on the osa-MIR528 precursor.Journal of Biotechnology,2012,160:146-15034.Chen LN,Yang Y,Yan CQ,Wang XM,Yu CL,Zhou J,Zhang WL,Cheng Y,Cheng XY,Chen JP*.Identification of Quantitative Trait Loci for Bacterial Blight Resistance Derived from Oryza meyeriana and Agronomic Traits in Recombinant Inbred Lines of Oryza sativa.Journal of Phytopathology,2012,160:461–46835.Guo WX,Wu GT,Yan F*,Lu YW,Zheng HY,Lin L,Chen HR,Chen JP*.Identification of Novel Oryza sativa miRNAs in Deep Sequencing-Based Small RNA Libraries of Rice Infected with Rice Stripe Virus.PLoS ONE,2012, 7( 10):e4644336.Yan F, Lu YW, Lin L, Zheng HY, Chen JP*.The Ability of PVX p25 to form RL structures in plant cells is necessary for its function in movement, but not for its suppression of RNA silencing.PLoS ONE,2012,7(8):e4324237.Yang Y, Xie L, Yan CQ, Wang XM, Yu CL, Cheng XY, Cheng Y, Chen JP*.Xylem Secondary Cell-wall Thickening Involved in Defense Responses of Oryza meyeriana to Xanthomonas oryzae pv. oryzae.Acta Phytopathologica Sinica,2012,42(5):505-51438.Zheng HY, Yan F, Lu YW, Sun LY, Lin L, Cai L, Hou MS, Chen JP*.Mapping of the functional domains of TuMV HC-Pro required for self- interaction and its subcellular localization.Virus Genes,2011,42:110-11639.Mo XH, Chen ZB, Chen JP*.Molecular identification and phylogenetic analysis of a viral RNA associated with the Chinese tobacco bushy top disease complex.Annals of Applied Biology,2011,158:188-19340.Yang Y, Yu CL, Wang XM, Yan CQ, Cheng Y, Chen JP*.Inoculation with Xanthomonas oryzae pv. oryzae induces thylakoid membrane association of Rubisco activase in Oryza meyeriana.Journal of Plant Physiology,2011,168:1701-170441.Lu YW, Yan F, Wei Guo, Zheng HY, Lin L, Peng JJ, Adams MJ, Chen JP*.Garlic virus X 11 kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus.Molecular Plant Pathology,2011,12(7):666–67642.Lin L, Luo ZP, Yan F, Lu YW, Zheng HY, Chen JP*.Interaction between potyvirus P3 and Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of host plants.Virus Genes,2011,43:90-9243.Li JS, Wang XM, Dong RX, Yang Y, Zhou J, Yu CL, Cheng Y, Yan CQ, Chen JP*.Evaluation of high-resolution melting for rice gene mapping.Plant Molecular Biology Reporter,2011,29:979-98544.Peng JJ, Yang J, Yan F*, Lu YW, Jiang SS, Lin L, Zheng HY, Chen HR, Chen JP*.Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana.Virus research,2011,158:268-27045.Hoang AT, Zhang HM, Yang J, Chen JP*, Hébrard E, Zhou GH, Vinh VN, Cheng JA.Identification, characterization, and distribution of Southern rice black-streaked dwarf virus in Vietnam.Plant Disease,2011,95(9):1063-106946.Wang JG, Zheng HY, Chen HR, Adams MJ, Chen JP*.Molecular diversities of Sugarcane mosaic virus and Sorghum mosaic virus isolates from Yunnan province, China.Journal of Phytopathology,2010,158:427-43247.Wang Q, Yang J, Zhou GH*, Zhang HM*, Chen JP, Adams MJ .The complete genome sequence of two isolates of Southern rice black-streaked dwarf virus, a new Fijivirus.Journal of Phytopathology,2010,158:733-73748.Yan F, Zhang HM, Adams MJ, Yang J, Peng JJ, Antoniw JF, Zhou YJ, Chen JP*.Characterization of siRNAs derived from Rice stripe virus in infected rice plants by deep sequencing.Archives of Virology,2010,155:935-94049.Wang XM, Yang Y, Yu CL, Cheng Y, Yan CQ, Chen JP*.A highly efficient method for construction of Rice artificial microRNA vectors.Molecular Biotechnology,2010,46(3):211-21850.Mo XH, Chen ZB, Chen JP*.Complete nucleotide sequence and genome organization of a Chinese isolate of tobacco vein distorting virus.Virus Genes,2010,41:425-43151.Yun T, Ni Z, Liu GQ, Yu B, Chen L, Huang JG, Zhang YM, Chen JP*.Generation of infectious and pathogenic Duck Hepatitis Virus Type 1 from cloned full-length cDNA .Virus Research,2010,147:159-165 科技奖励截至2018年11月,陈剑平先后获得国家科技进步奖一等奖1项、二等奖4项(均为第一完成人),部省科技进步奖一等奖8项、二等奖5项
时间奖励项目所获奖励完成人排名1986年小麦黄花叶病的研究浙江省科学技术进步奖三等奖第三完成人1991年三种真菌传大小麦病毒血清学生物化学和传毒介体的研究浙江省科学技术进步奖三等奖第二完成人1992年大麦和性花叶病毒在其禾谷多黏菌介体内的发现和增殖的研究农业部科技进步然一等奖第一完成人1993年主要粮食作物病毒理化特性、抗源鉴定和防治农业部科学技术进步三等奖第九完成人1995年大麦和性花叶病毒在其禾谷多黏菌介体内的发现和增殖的研究国家科技进步奖一等奖第一完成人1998年土传小麦花叶病毒基因组(RNA2)遗传不稳定性和分子变异的研究农业部科技进步奖一等奖第一完成人2000年我国大麦黄花叶病毒株系鉴定、抗源筛选、抗病品种应用及其分子生物学研究浙江省科技进步奖一等奖第一完成人2001年我国大麦黄花叶病毒株系鉴定、抗源筛选、抗病品种应用及其分子生物学研究国家科技进步奖二等奖第一完成人2001年真菌传麦类棒状病毒新种类、血清学特性及其基因组全序列研究浙江省科技进步奖二等奖第一完成人2002年禾谷多黏菌传小麦线状病毒种类、发病规律、抗源筛选及其分子生物学研究浙江省科技进步奖一等奖第一完成人2003年我国水稻黑条矮缩病病原、成灾原因和持续控制技术浙江省科技进步奖二等奖第二完成人2004年我国水稻黑条矮缩病和玉米粗缩病病原、发生规律及其持续控制技术国家科技进步奖二等奖第一完成人2004年禾谷多黏菌生态学、超微结构及其传播的燕麦花叶病毒基因组全序列研究浙江省科技进步奖一等奖第一完成人2005年禾谷多黏菌传小麦线状病毒种类、发病规律、抗源筛选及其分子生物学研究国家科学技术奖二等奖第一完成人2005年禾谷多黏菌及其传播的小麦病毒种类、发生规律和综合防治技术应用国家科技进步奖二等奖第一完成人2006年水稻抗真菌(稻瘟病)的基因工程浙江省科技进步奖二等奖第四完成人2007年我国50种植物病毒分子鉴定及其基因组研究浙江省科学技术一等奖第一完成人2008年水稻条纹叶枯病与介体灰飞虱发生规律、监测预警与持续控制技术浙江省科学技术一等奖第二完成人2008年长叶车前草花叶病毒侵染性克隆构建及外源基因表达研究浙江省科技进步奖二等奖第一完成人
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